Amounts shown.
Every active ingredient and its amount is listed.
Our standards
Built for people who ask why.
We make the formula easier to check.
See every active amount, every named probiotic strain and the reason each ingredient earned its place.
Strains named.
Every probiotic includes its full strain name.
Each ingredient has a job.
Every ingredient supports one of the five GX-5™ roles.
Research connected.
Follow each ingredient to the human research behind its role.
Inside GX-5™
Every ingredient.
Nothing hidden.
See what each ingredient does, why it matters in daily life and how its disclosed amount fits into the complete GX-5™ system.
Fortify · Gut lining
L-Glutamine
1,900 mg per daily serve
A foundational amino acid chosen to support the intestinal lining—the hardworking barrier between what you eat and the rest of your body.
What it is
L-glutamine is an amino acid the body naturally makes and uses throughout the intestinal lining.
Why it earned its place
Gut health is more than the microbiome. L-glutamine gives GX-5™ a dedicated ingredient for the physical lining, so Fortify supports the barrier as well as the bacteria around it.
How it works in GX-5™
It anchors Fortify beside N-acetyl glucosamine and zinc—three complementary ingredients built around one clear role.
Formula role: A fully disclosed 1,900 mg foundation for the Fortify layer.
Fortify · Gut lining
N-Acetyl Glucosamine
300 mg per daily serve
A considered building block selected with the gut’s protective mucus layer in mind.
What it is
N-acetyl glucosamine is an amino sugar related to compounds naturally present in mucus and connective tissue.
Why it earned its place
The mucus layer helps form the gut’s first protective surface. NAG gives Fortify a distinct structural role that probiotics and fibre do not provide.
How it works in GX-5™
Paired with L-glutamine and zinc, it helps make Fortify a layered blend rather than a single-ingredient gesture.
Formula role: 300 mg, fully disclosed, corn-derived and selected for our vegan formula.
Fortify · Gut lining
Zinc Gluconate
15 mg per daily serve
An essential mineral chosen to support normal cell renewal and the tissues that maintain protective barriers.
What it is
Zinc is an essential mineral the body needs but cannot make. Zinc gluconate is the considered form used here.
Why it earned its place
Your gut lining renews constantly. Zinc earns its place because normal zinc nutrition supports the cell and tissue processes behind that everyday maintenance.
How it works in GX-5™
It completes the Fortify trio with L-glutamine and N-acetyl glucosamine, so no single ingredient is asked to do everything.
Formula role: 15 mg, fully disclosed, completing the three-part Fortify layer.
Flora · Named probiotic strain
S. boulardii CNCM I-745
3 billion CFU per daily serve
A named probiotic yeast selected to support microbiome resilience when everyday life puts digestion under pressure.
What it is
S. boulardii is a probiotic yeast. CNCM I-745 identifies the exact strain, not only the species.
Why it earned its place
Exact-strain outcome: In a human study during antibiotic use, CNCM I-745 helped limit some disruption to the gut microbiome and reduced diarrhoea scores. Put simply, this strain was chosen for support when the gut is under pressure.
Why the full name matters
Probiotic findings belong to the strain tested. Naming CNCM I-745 makes the choice traceable.
Formula role: 3 billion CFU from a named strain within a 10-billion-CFU blend.
Flora · Named probiotic strain
B. coagulans BC30
5 billion CFU per daily serve
A resilient named probiotic chosen with everyday regularity and digestive comfort in mind.
What it is
BC30 is a spore-forming probiotic. Its natural protective structure is well suited to a stable daily powder.
Why it earned its place
Exact-strain outcome: In a four-week placebo-controlled study, 1 billion CFU of BC30 daily helped adults go more regularly, improved stool consistency and reduced reports of constipation. GX-5™ provides 5 billion CFU per daily serve.
How it works in GX-5™
At 5 billion CFU, BC30 forms the largest share of the three-strain Flora blend.
Formula role: 5 billion CFU from a stable, named strain with human gut research.
Flora · Named probiotic strain
B. subtilis DE111
2 billion CFU per daily serve
A resilient probiotic strain selected to support everyday digestive balance.
What it is
DE111 is a named, spore-forming probiotic strain. Its naturally protective structure helps it remain stable in a daily powder and survive the journey through the digestive system.
Why it earned its place
Exact-strain outcome: In a 90-day placebo-controlled study, 1 billion CFU of DE111 daily helped move stool form toward a more normal range in adults with occasional irregularity. GX-5™ provides 2 billion CFU per daily serve.
How it works in GX-5™
DE111 works alongside S. boulardii CNCM I-745 and B. coagulans BC30, creating a complementary three-strain blend supported by prebiotic fibre.
Formula role: 2 billion CFU from a named probiotic strain, contributing to the complete 10 billion CFU Flora blend.
Nourish · Prebiotic fibre
Acacia Gum · FiberGum B
3,000 mg per daily serve
A soluble plant fibre that nourishes beneficial gut bacteria and helps make fibre support a meaningful daily habit.
What it is
Acacia gum is a soluble fibre from the acacia tree. FiberGum B is the branded form selected for The Daily Buffer™.
Why it earned its place
Adding probiotics is only half the story. FiberGum B provides fermentable fibre the microbiome can use, while building half of the substantial 6 g Nourish layer.
How it works in GX-5™
Three grams of acacia meets three grams of PHGG to create a substantial dual-fibre layer.
Formula role: 3 g of branded acacia fibre—half of the 6 g Nourish layer.
Nourish · Prebiotic fibre
PHGG · Fibalance
3,000 mg per daily serve
A soluble, non-gelling prebiotic fibre chosen for regularity, digestive comfort and a drink people will actually want to take every day.
What it is
PHGG stands for partially hydrolysed guar gum. It dissolves without turning your daily drink into a thick gel.
Why it earned its place
PHGG brings both function and usability: human studies have explored stool form, bloating and microbiome outcomes, while its low viscosity keeps the daily serve easy to drink.
How it works in GX-5™
Three grams of PHGG meets three grams of acacia fibre, combining two complementary fibre sources.
Formula role: 3 g of branded PHGG—completing the 6 g Nourish layer.
Shield · Targeted plant compound
Black Seed Oil Powder
500 mg per daily serve
A concentrated botanical chosen to add a distinct plant compound to the Shield layer.
What it is
Black seed comes from Nigella sativa. Its oil contains thymoquinone, a naturally occurring plant compound studied in human research.
Why it earned its place
Black seed has a long history of food use and a growing body of human research. It gives Shield a clear botanical role alongside quercetin.
How it works in GX-5™
Black seed brings an oil-based botanical. Quercetin adds a complementary flavonoid found naturally in food.
Formula role: 500 mg of black seed oil powder, paired deliberately within Shield.
Shield · Targeted plant compound
Quercetin Dihydrate
150 mg per daily serve
A food-derived flavonoid selected to complement black seed, while naturally giving The Daily Buffer™ its bright yellow colour.
What it is
Quercetin is found naturally in foods including apples, onions and berries. Dihydrate is the stable form used here.
Why it earned its place
Quercetin earns its place through formula breadth: it brings a distinct flavonoid found in familiar plant foods, so Shield is a complementary pair rather than a single botanical.
How it works in GX-5™
Paired with black seed, it creates a measured two-part botanical layer with two distinct plant-compound profiles.
Formula role: 150 mg, fully disclosed, adding a purposeful food-derived flavonoid to Shield.
Flow · Digestive enzymes
Five-Enzyme Blend
Five targeted enzymes chosen to help break down the protein, fats, carbohydrates, lactose and plant fibre in the mixed meals people actually eat.
What enzymes do
Digestive enzymes help break larger food molecules into smaller parts. Different enzymes work on different foods.
Why five
A mixed meal rarely asks the gut to handle just one thing. Five enzymes give Flow practical coverage across the protein, fats, carbohydrates, lactose and plant fibre people commonly eat together.
Why activity units matter
Weight tells you how much powder is present. Activity units tell you what each enzyme can do, so we disclose every one.
Amylase8,000 DU · carbohydrates
Protease35,000 HUT · protein
Lipase750 FIP · fats
Cellulase500 CU · plant fibre
Lactase1,500 ALU · dairy and lactose
Formula role: Five declared enzymes for five major parts of a mixed meal.
11 formula cards shown
Amounts shown are per daily serve. Explore the research library for the human studies connected to each ingredient or named strain.
The Gut Theory Research Library
Built as a formula.
Backed by context.
We chose eleven ingredients to work together in one daily formula. Below, we show why each one earned its place and when a study used a different amount, ingredient source or group of people. These papers do not test The Daily Buffer™ as a finished product.
Study resultWhat changed for the people who took part.
Match to our formulaSee whether the study tested the exact ingredient or probiotic strain used in GX-5™.
Amount and peopleHow much was used, who took part and how long the study lasted.
PHGG · related ingredient research
Stool form and the gut microbiome.
In a three-month placebo-controlled study, 5 g of PHGG daily helped move stool form toward normal and increased Bifidobacterium in 44 adults prone to loose stools.
Product context: The study used a different branded PHGG. The Daily Buffer™ contains 3 g of Fibalance PHGG within its 6 g dual fibre blend. It did not test our ingredient source, amount or finished product.
Open the original paper ↗BC30 · direct strain outcome
BC30 and regularity.
In 111 adults with everyday gut complaints, 1 billion CFU of BC30 daily for four weeks improved stool frequency, stool consistency and constipation measures versus placebo. The Daily Buffer provides 5 billion CFU of the same named strain.
Open the original paper ↗PHGG · related ingredient research
Bloating and gas in people with IBS.
Across 12 weeks, 6 g of PHGG a day improved bloating and gas compared with placebo in adults with IBS.
Product context: The study used a different branded PHGG. The Daily Buffer™ contains 3 g of Fibalance PHGG within its 6 g dual fibre blend. It did not test our ingredient source, amount or finished product.
Open the original paper ↗Acacia fibre · related ingredient research
Acacia fibre and stool frequency.
In a four week placebo controlled study, adults with constipation predominant IBS took 10 g of acacia fibre a day. The study reported an increase in stool frequency.
Product context: The study used a different branded acacia fibre. The Daily Buffer™ contains 3 g of FiberGum B within its 6 g dual fibre blend. It did not test our ingredient source, amount or finished product.
Open the original paper ↗L-glutamine · different amount and study group
L-glutamine in a specific study group.
This eight week study tested 15 g of L-glutamine a day in adults with post infectious IBS-D and increased gut permeability. It reported changes in symptoms, stool measures and permeability compared with placebo.
Product context: The Daily Buffer™ contains 1.9 g of L-glutamine per daily serve. This study did not test our amount, intended customer or finished product.
Open the original paper ↗Zinc gluconate · different amount
Zinc and gut barrier markers.
This small study used separate groups to examine intestinal tissue and blood markers. Participants received zinc gluconate providing 52 mg of zinc a day for tissue tests. A separate group received 60 mg a day for a blood marker.
Product context: The Daily Buffer™ lists 15 mg of zinc gluconate per daily serve. This study used higher amounts for short term marker tests and did not test our listed amount or finished product.
Open the original paper ↗CNCM I-745 · situational outcome
S. boulardii during antibiotic use.
In adults taking an antibiotic, S. boulardii CNCM I-745 reduced some antibiotic-linked microbiome changes and diarrhoea scores. It is the exact named strain used in The Daily Buffer, at 3 billion CFU per serve.
Open the original paper ↗DE111 · early strain evidence
DE111 and stool form.
Across 90 days, adults taking 1 billion CFU of DE111 reported more normal stool forms than those taking placebo. The Daily Buffer provides 2 billion CFU of the same named strain.
Open the original paper ↗Five enzymes · related formulation
A five-enzyme complex and dyspepsia.
In a 60-day placebo-controlled study, a related five-enzyme complex improved functional dyspepsia symptom scores. It used the same five enzyme types found in Flow; The Daily Buffer separately discloses the activity of every enzyme.
Open the original paper ↗Black seed oil · human and early laboratory research
Black seed oil and digestive research.
A double-blind, placebo-controlled study followed 70 adults with functional dyspepsia for eight weeks. Those taking 5 mL of black seed oil daily with honey had improved digestive symptom scores compared with those receiving honey alone.
Product context: Both groups received honey, so the main difference was the black seed oil. The study used a higher amount in people with a diagnosed digestive condition. The Daily Buffer™ contains 500 mg of black seed oil powder and was not tested in this study.
Early microbiome research: In a 2025 laboratory study, black cumin seed extract increased the production of acetate, propionate and butyrate by microbes collected from human stool samples. This work was performed outside the human body and tested a different extract, so it does not show that The Daily Buffer™ changes the human gut microbiome.
10 research entries shown
Research shown relates to individual ingredients, named strains or clearly labelled related formulations in the people and amounts studied. This library is general education, not medical advice.